Sulfotransferases in Biosynthesis of Glycosaminoglycan.
نویسندگان
چکیده
منابع مشابه
Investigating the elusive mechanism of glycosaminoglycan biosynthesis.
Glycosaminoglycan (GAG) biosynthesis requires numerous biosynthetic enzymes and activated sulfate and sugar donors. Although the sequence of biosynthetic events is resolved using reconstituted systems, little is known about the emergence of cell-specific GAG chains (heparan sulfate, chondroitin sulfate, and dermatan sulfate) with distinct sulfation patterns. We have utilized a library of click-...
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We have developed a rapid, high throughput screening assay for compounds that alter the assembly of glycosaminoglycan chains in Chinese hamster ovary cells. The assay uses autoradiography to measure the binding of newly synthesized [35S]proteoglycans and [35S]glycosaminoglycans to a positively charged membrane. Screening over 1000 extracts from a random plant collection obtained from the Amazon...
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Heparan sulfate/heparin (HS/HP) is a large complex carbohydrate and binds various proteins, such as FGF, TGFβ/Dpp, Wnt/Wingless, Hedgehog and anti-thrombin III. In the biosynthesis of HS/HP, N-deacetylation and N-sulfation of N-acetyl glucosamine are catalyzed by a single polypeptide, NDST, and have important roles in forming unique sequences for binding those proteins. Four NDST isozymes are f...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 1995
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.7.527